The Human Immune Response to Dengue Virus Is Dominated by Highly Cross-Reactive Antibodies Endowed with Neutralizing and Enhancing Activity
Identifieur interne : 006E71 ( Main/Exploration ); précédent : 006E70; suivant : 006E72The Human Immune Response to Dengue Virus Is Dominated by Highly Cross-Reactive Antibodies Endowed with Neutralizing and Enhancing Activity
Auteurs : Martina Beltramello [Suisse] ; Katherine L. Williams [États-Unis] ; Cameron P. Simmons ; Annalisa Macagno [Suisse] ; Luca Simonelli [Suisse] ; Nguyen Than Ha Quyen ; Soila Sukupolvi-Petty [États-Unis] ; Erika Navarro-Sanchez [France] ; Paul R. Young [Australie] ; Aravinda M. De Silva [États-Unis] ; Félix A. Rey [France] ; Luca Varani [Suisse] ; Stephen S. Whitehead [États-Unis] ; Michael S. Diamond [États-Unis] ; Eva Harris [États-Unis] ; Antonio Lanzavecchia [Suisse] ; Federica Sallusto [Suisse]Source :
- Cell host & microbe [ 1931-3128 ] ; 2010.
Abstract
Antibodies protect against homologous Dengue virus (DENV) infection but can precipitate severe dengue by promoting heterotypic virus entry via Fcγ receptors (FcγR). We immortalized memory B cells from individuals after primary or secondary infection and analyzed anti-DENV monoclonal antibodies (mAbs) thus generated. MAbs to envelope (E) protein domain III (DIII) were either serotype specific or cross-reactive and potently neutralized DENV infection. DI/DII- or viral membrane protein prM-reactive mAbs neutralized poorly and showed broad cross-reactivity with the four DENV serotypes. All mAbs enhanced infection at subneutralizing concentrations. Three mAbs targeting distinct epitopes on the four DENV serotypes and engineered to prevent FcγR binding did not enhance infection and neutralized DENV
Url:
DOI: 10.1016/j.chom.2010.08.007
PubMed: 20833378
PubMed Central: 3884547
Affiliations:
- Australie, France, Suisse, États-Unis
- Californie, Caroline du Nord, Maryland, Missouri (État), Île-de-France
- Saint-Louis (Missouri)
- École de médecine (Université Washington de Saint-Louis)
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Williams</name><affiliation wicri:level="2"><nlm:aff id="A2">Division of Infectious Diseases, University of California, Berkeley, CA 94720-7354, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Division of Infectious Diseases, University of California, Berkeley, CA 94720-7354</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Simmons, Cameron P" sort="Simmons, Cameron P" uniqKey="Simmons C" first="Cameron P." last="Simmons">Cameron P. 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Williams</name><affiliation wicri:level="2"><nlm:aff id="A2">Division of Infectious Diseases, University of California, Berkeley, CA 94720-7354, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Division of Infectious Diseases, University of California, Berkeley, CA 94720-7354</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Simmons, Cameron P" sort="Simmons, Cameron P" uniqKey="Simmons C" first="Cameron P." last="Simmons">Cameron P. Simmons</name><affiliation><nlm:aff id="A3">Hospital for Tropical Diseases, Oxford University Clinical Research Unit, Ho Chi Minh City Viet Nam</nlm:aff><wicri:noCountry code="subfield">Ho Chi Minh City Viet Nam</wicri:noCountry></affiliation></author><author><name sortKey="Macagno, Annalisa" sort="Macagno, Annalisa" uniqKey="Macagno A" first="Annalisa" last="Macagno">Annalisa Macagno</name><affiliation wicri:level="1"><nlm:aff id="A1">Institute for Research in Biomedicine, Bellinzona, 6500, Switzerland</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea>Institute for Research in Biomedicine, Bellinzona, 6500</wicri:regionArea><wicri:noRegion>6500</wicri:noRegion></affiliation></author><author><name sortKey="Simonelli, Luca" sort="Simonelli, Luca" uniqKey="Simonelli L" first="Luca" last="Simonelli">Luca Simonelli</name><affiliation wicri:level="1"><nlm:aff id="A1">Institute for Research in Biomedicine, Bellinzona, 6500, Switzerland</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea>Institute for Research in Biomedicine, Bellinzona, 6500</wicri:regionArea><wicri:noRegion>6500</wicri:noRegion></affiliation></author><author><name sortKey="Quyen, Nguyen Than Ha" sort="Quyen, Nguyen Than Ha" uniqKey="Quyen N" first="Nguyen Than Ha" last="Quyen">Nguyen Than Ha Quyen</name><affiliation><nlm:aff id="A3">Hospital for Tropical Diseases, Oxford University Clinical Research Unit, Ho Chi Minh City Viet Nam</nlm:aff><wicri:noCountry code="subfield">Ho Chi Minh City Viet Nam</wicri:noCountry></affiliation></author><author><name sortKey="Sukupolvi Petty, Soila" sort="Sukupolvi Petty, Soila" uniqKey="Sukupolvi Petty S" first="Soila" last="Sukupolvi-Petty">Soila Sukupolvi-Petty</name><affiliation wicri:level="4"><nlm:aff id="A4">Departments of Medicine, Molecular Microbiology, Pathology & Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Departments of Medicine, Molecular Microbiology, Pathology & Immunology, Washington University School of Medicine, St. Louis, MO 63110</wicri:regionArea><placeName><region type="state">Missouri (État)</region><settlement type="city">Saint-Louis (Missouri)</settlement></placeName><orgName type="university">École de médecine (Université Washington de Saint-Louis)</orgName></affiliation></author><author><name sortKey="Navarro Sanchez, Erika" sort="Navarro Sanchez, Erika" uniqKey="Navarro Sanchez E" first="Erika" last="Navarro-Sanchez">Erika Navarro-Sanchez</name><affiliation wicri:level="1"><nlm:aff id="A5">Department of Virology, Institute Pasteur, Paris, 75724, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Department of Virology, Institute Pasteur, Paris, 75724</wicri:regionArea><wicri:noRegion>75724</wicri:noRegion><wicri:noRegion>75724</wicri:noRegion></affiliation></author><author><name sortKey="Young, Paul R" sort="Young, Paul R" uniqKey="Young P" first="Paul R." last="Young">Paul R. Young</name><affiliation wicri:level="1"><nlm:aff id="A6">Centre for Infectious Disease Research, School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, Queensland 4072, Australia</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Centre for Infectious Disease Research, School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, Queensland 4072</wicri:regionArea><wicri:noRegion>Queensland 4072</wicri:noRegion></affiliation></author><author><name sortKey="De Silva, Aravinda M" sort="De Silva, Aravinda M" uniqKey="De Silva A" first="Aravinda M." last="De Silva">Aravinda M. De Silva</name><affiliation wicri:level="2"><nlm:aff id="A7">Department of Microbiology and Immunology, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology and Immunology, University of North Carolina School of Medicine, Chapel Hill, NC 27599</wicri:regionArea><placeName><region type="state">Caroline du Nord</region></placeName></affiliation></author><author><name sortKey="Rey, Felix A" sort="Rey, Felix A" uniqKey="Rey F" first="Félix A." last="Rey">Félix A. Rey</name><affiliation wicri:level="3"><nlm:aff id="A5">Department of Virology, Institute Pasteur, Paris, 75724, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Department of Virology, Institute Pasteur, Paris, 75724</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region></placeName></affiliation></author><author><name sortKey="Varani, Luca" sort="Varani, Luca" uniqKey="Varani L" first="Luca" last="Varani">Luca Varani</name><affiliation wicri:level="1"><nlm:aff id="A1">Institute for Research in Biomedicine, Bellinzona, 6500, Switzerland</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea>Institute for Research in Biomedicine, Bellinzona, 6500</wicri:regionArea><wicri:noRegion>6500</wicri:noRegion></affiliation></author><author><name sortKey="Whitehead, Stephen S" sort="Whitehead, Stephen S" uniqKey="Whitehead S" first="Stephen S." last="Whitehead">Stephen S. Whitehead</name><affiliation wicri:level="2"><nlm:aff id="A8">Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institute of Health, Bethesda, MD 20892-8133, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institute of Health, Bethesda, MD 20892-8133</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Diamond, Michael S" sort="Diamond, Michael S" uniqKey="Diamond M" first="Michael S." last="Diamond">Michael S. Diamond</name><affiliation wicri:level="4"><nlm:aff id="A4">Departments of Medicine, Molecular Microbiology, Pathology & Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Departments of Medicine, Molecular Microbiology, Pathology & Immunology, Washington University School of Medicine, St. Louis, MO 63110</wicri:regionArea><placeName><region type="state">Missouri (État)</region><settlement type="city">Saint-Louis (Missouri)</settlement></placeName><orgName type="university">École de médecine (Université Washington de Saint-Louis)</orgName></affiliation></author><author><name sortKey="Harris, Eva" sort="Harris, Eva" uniqKey="Harris E" first="Eva" last="Harris">Eva Harris</name><affiliation wicri:level="2"><nlm:aff id="A2">Division of Infectious Diseases, University of California, Berkeley, CA 94720-7354, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Division of Infectious Diseases, University of California, Berkeley, CA 94720-7354</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Lanzavecchia, Antonio" sort="Lanzavecchia, Antonio" uniqKey="Lanzavecchia A" first="Antonio" last="Lanzavecchia">Antonio Lanzavecchia</name><affiliation wicri:level="1"><nlm:aff id="A1">Institute for Research in Biomedicine, Bellinzona, 6500, Switzerland</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea>Institute for Research in Biomedicine, Bellinzona, 6500</wicri:regionArea><wicri:noRegion>6500</wicri:noRegion></affiliation></author><author><name sortKey="Sallusto, Federica" sort="Sallusto, Federica" uniqKey="Sallusto F" first="Federica" last="Sallusto">Federica Sallusto</name><affiliation wicri:level="1"><nlm:aff id="A1">Institute for Research in Biomedicine, Bellinzona, 6500, Switzerland</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea>Institute for Research in Biomedicine, Bellinzona, 6500</wicri:regionArea><wicri:noRegion>6500</wicri:noRegion></affiliation></author></analytic><series><title level="j">Cell host & microbe</title><idno type="ISSN">1931-3128</idno><idno type="eISSN">1934-6069</idno><imprint><date when="2010">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>Summary</title><p id="P1">Antibodies protect against homologous Dengue virus (DENV) infection but can precipitate severe dengue by promoting heterotypic virus entry via Fcγ receptors (FcγR). We immortalized memory B cells from individuals after primary or secondary infection and analyzed anti-DENV monoclonal antibodies (mAbs) thus generated. MAbs to envelope (E) protein domain III (DIII) were either serotype specific or cross-reactive and potently neutralized DENV infection. DI/DII- or viral membrane protein prM-reactive mAbs neutralized poorly and showed broad cross-reactivity with the four DENV serotypes. All mAbs enhanced infection at subneutralizing concentrations. Three mAbs targeting distinct epitopes on the four DENV serotypes and engineered to prevent FcγR binding did not enhance infection and neutralized DENV <italic>in vitro</italic> and <italic>in vivo</italic> as postexposure therapy in a mouse model of lethal DENV infection. Our findings reveal an unexpected degree of cross-reactivity in human antibodies against DENV and illustrate the potential for an antibody-based therapy to control severe dengue.</p></div></front></TEI><affiliations><list><country><li>Australie</li><li>France</li><li>Suisse</li><li>États-Unis</li></country><region><li>Californie</li><li>Caroline du Nord</li><li>Maryland</li><li>Missouri (État)</li><li>Île-de-France</li></region><settlement><li>Saint-Louis (Missouri)</li></settlement><orgName><li>École de médecine (Université Washington de Saint-Louis)</li></orgName></list><tree><noCountry><name sortKey="Quyen, Nguyen Than Ha" sort="Quyen, Nguyen Than Ha" uniqKey="Quyen N" first="Nguyen Than Ha" last="Quyen">Nguyen Than Ha Quyen</name><name sortKey="Simmons, Cameron P" sort="Simmons, Cameron P" uniqKey="Simmons C" first="Cameron P." last="Simmons">Cameron P. Simmons</name></noCountry><country name="Suisse"><noRegion><name sortKey="Beltramello, Martina" sort="Beltramello, Martina" uniqKey="Beltramello M" first="Martina" last="Beltramello">Martina Beltramello</name></noRegion><name sortKey="Lanzavecchia, Antonio" sort="Lanzavecchia, Antonio" uniqKey="Lanzavecchia A" first="Antonio" last="Lanzavecchia">Antonio Lanzavecchia</name><name sortKey="Macagno, Annalisa" sort="Macagno, Annalisa" uniqKey="Macagno A" first="Annalisa" last="Macagno">Annalisa Macagno</name><name sortKey="Sallusto, Federica" sort="Sallusto, Federica" uniqKey="Sallusto F" first="Federica" last="Sallusto">Federica Sallusto</name><name sortKey="Simonelli, Luca" sort="Simonelli, Luca" uniqKey="Simonelli L" first="Luca" last="Simonelli">Luca Simonelli</name><name sortKey="Varani, Luca" sort="Varani, Luca" uniqKey="Varani L" first="Luca" last="Varani">Luca Varani</name></country><country name="États-Unis"><region name="Californie"><name sortKey="Williams, Katherine L" sort="Williams, Katherine L" uniqKey="Williams K" first="Katherine L." last="Williams">Katherine L. Williams</name></region><name sortKey="De Silva, Aravinda M" sort="De Silva, Aravinda M" uniqKey="De Silva A" first="Aravinda M." last="De Silva">Aravinda M. De Silva</name><name sortKey="Diamond, Michael S" sort="Diamond, Michael S" uniqKey="Diamond M" first="Michael S." last="Diamond">Michael S. Diamond</name><name sortKey="Harris, Eva" sort="Harris, Eva" uniqKey="Harris E" first="Eva" last="Harris">Eva Harris</name><name sortKey="Sukupolvi Petty, Soila" sort="Sukupolvi Petty, Soila" uniqKey="Sukupolvi Petty S" first="Soila" last="Sukupolvi-Petty">Soila Sukupolvi-Petty</name><name sortKey="Whitehead, Stephen S" sort="Whitehead, Stephen S" uniqKey="Whitehead S" first="Stephen S." last="Whitehead">Stephen S. Whitehead</name></country><country name="France"><noRegion><name sortKey="Navarro Sanchez, Erika" sort="Navarro Sanchez, Erika" uniqKey="Navarro Sanchez E" first="Erika" last="Navarro-Sanchez">Erika Navarro-Sanchez</name></noRegion><name sortKey="Rey, Felix A" sort="Rey, Felix A" uniqKey="Rey F" first="Félix A." last="Rey">Félix A. Rey</name></country><country name="Australie"><noRegion><name sortKey="Young, Paul R" sort="Young, Paul R" uniqKey="Young P" first="Paul R." last="Young">Paul R. Young</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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